The pair have each been charged with murder and attempting to defeat the ends of justice.
The suspects, both of Greenock, made no plea to the two separate charges last week and were remanded in custody ahead of their next court appearance.
A 45-year-old man previously arrested in connection with assault and a 41-year-old man arrested at the same time as the two women have both been released pending further enquiries.
Police Scotland previously said officers investigating Mr Best’s death were carrying out enquiries into a report of a disturbance in Lansbury Street, Greenock, which took place between 11pm on 10 November and 3am on 11 November.
A force spokesperson said: “Enquiries are ongoing.”