A new study, 'Sensitive poliovirus detection using nested PCR and nanopore sequencing: a prospective validation study', published today (17 August 2023) in Nature Microbiology, proves that using DDNS to detect polio outbreaks can save public health authorities crucial time and money.
This research was jointly conducted by researchers at the Institut National de Recherche Biomédicale in Kinshasa who implemented DDNS in the Democratic Republic of the Congo (DRC) for the detection of polio outbreaks in collaboration with the MHRA, Imperial College London, the University of Edinburgh and various laboratories of the World Health Organization (WHO) Global Polio Laboratory Network (GPLN), with support from the Bill and Melinda Gates Foundation.
This is the first time that this type of scientific technique has been used to detect polio. Similar techniques have previously been used to detect COVID-19, Ebola, measles and monkeypox.
By enabling samples to be tested in the country where the outbreak originated rather than being sent to specialist laboratories abroad, the costs and delays of transport and testing can be reduced from an average of 42 days to an average of 19 days.
Currently, stool samples from countries with active polio outbreaks such as the DRC must be shipped around the world for lengthy, complex laboratory tests to confirm a polio case. Faster detection of polio in the regions where outbreaks still occur allows for a faster response by authorities through targeted, localised vaccination campaigns, minimising the opportunity for the virus to spread.
Javier Martin, Principal Scientist in Virology at the MHRA said: We are standing at a delicate and pivotal moment for the eradication of polio. While vaccination programmes have seen polio disappear in many countries, the delayed detection of outbreaks poses a major threat to those efforts.
By implementing detection methods such as DDNS, we can identify where outbreaks are and which polio strain is present much more quickly, allowing us to act at the earliest opportunity.
This is the result of years of work, collaborating with our partners. Together, we will continue to build on this research and support countries at risk of outbreaks to implement DDNS testing to help make polio a disease of the past."
This research showed that DDNS tests done locally in the DRC over a six-month period were an average of 23 days faster than the standard method, with over 99% accuracy.
Researchers also tested this technique in the UK and detected poliovirus in London in 2022, leading to the recent drive to ensure children under the age of 12 are vaccinated through the London polio catch-up campaign 2023.
Professor Placide Mbala-Kingebeni, Medical Doctor and Virologist at the Institut National de Recherche Biomédicale said: This is the perfect example of collaboration, where combining and sharing knowledge together with all our partners has supported the vital work of the INRB in the DRC where poliomyelitis remains a serious public health problem.
Collaboration and training with our partners has empowered the local team not only to master and confidently carry out this new technique but also to transfer the knowledge and skills to other African countries where poliovirus outbreaks are reported regularly.
The support and guidance of the Bill and Melinda Gates Foundation and the GPLN, who make these collaborations possible, enables the application and expansion of DDNS across Africa for the rapid detection of poliovirus and outbreak response, helping us move closer towards polio eradication."
Dr Alex Shaw, Research Fellow in the School of Public Health at Imperial College London said: This method allows the rapid confirmation of polio strains, facilitating swifter vaccine responses that can reduce the number of polio cases stemming from an outbreak. Development and validation of the method has been the result of fruitful collaboration between a consortium of many partners.
As a consortium we look forward to the training of additional national laboratories in this method, with prior trainees, including members of INRB, now taking on the role of trainers.
The sequencing technology used in this method is easily adapted for the detection and typing of other organisms. This rollout will therefore provide a foundation of skills and experience that can be redirected to the genomic surveillance of other pathogens as needed." Related StoriesCutting-edge research: machine learning identifies early predictors of type 1 diabetesBibliometric analysis reveals research trends connecting Alzheimer's disease and the gut microbiomeResearch uncovers new insights into post-COVID-19 syndrome (PCS) phenotypes and impact on quality of life
Polio is an infectious disease caused by the poliovirus, most commonly transmitted through contact with infected feces via contaminated food and water.
While many people may never show symptoms, in extreme cases, especially for babies and children under the age of five, polio can lead to permanent paralysis or death.
The WHO has identified delays in detection as one of the major challenges facing their Polio eradication strategy 2022-2026.
While faster detection methods such as DDNS cannot eradicate polio on their own, they play an essential part in managing outbreaks.
Scientists at the MHRA will continue to support the testing and validation of DDNS as a polio detection technique and training WHO laboratories around the world in how to use it. Source:
GOV.UKJournal reference:
Shaw, A. G., et al. (2023). Sensitive poliovirus detection using nested PCR and nanopore sequencing: a prospective validation study. Nature Microbiology. doi.org/10.1038/s41564-023-01453-4.
Social media accounts expressing support for a Pakistan-based terror group linked to al Qaeda appear to have posted recent videos from a Pakistan mosque targeted by Indian airstrikes.
Sky News has found videos posted on TikTok, YouTube and Google that appear to be filmed at the Markaz Taiba Mosque in Muridke. The captions and usernames contain expressions of support for the Lashkar-e-Taiba (LeT) and a group called ‘313’.
Sky News has found and geolocated multiple videos that appear to be filmed in the area where the captions include either or both ‘313’ and LeT.
Some of the videos show men in the streets with guns. Another post captioned a video of children doing martial arts training inside the targeted mosque, “we are little soldiers, and we fight the non-believers”.
Image: The caption of the video reads ‘we are little soldiers, and we fight the non-believers’. It uses the hashtag ‘313’.
The caption uses the hashtag #جہاد313, which translates to ‘313’ jihad.
‘313’ appears to refer to the 313 Brigade, a proscribed terror organisation in Pakistan.
In a TikTok video posted to the Google page for Markaz Taiba Mosque in Muridke, a man can be seen walking along the street with a gun.
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The account that posted that video wrote in their description, “Lashkar Taiba, Mujahid Force, ‘313’ and Markaz Taiba Muridke”, self-proclaiming their support for the groups.
Image: This screenshot from the Google user labels Lashkar-e-Taiba and ‘313’ and includes the location name Muridke
Gunmen opened fire on tourists, killing 26 people and injuring dozens in a popular holiday spot near Pahalgam, Kashmir, on 22 April.
LeT were accused by India of involvement in the Pahalgam attack through their proxy the Resistance Front, which claimed responsibility for the attack.
LeT, which is designated as a terrorist organisation by the UN Security Council and the UK, focuses on fighting Indian control in Kashmir and is based in the Punjab region of Pakistan.
Pakistan denies allegations of terror camps operating in the country. This region has been in the control of the Punjabi government since 2010. The Punjab government condemned the Indian strikes, and declared a state of emergency across Punjab.
Muskan Sangwan, senior intelligence analyst at TRAC, a terrorism research and analysis consortium, told Sky News: “Brigade 313 is al Qaeda in Pakistan. It’s an umbrella organisation for members of several groups like Taliban, Lashkar-e-Jhangvi, Haqqat ul-Jihad-al-Islami, Jaish-e Muhammad, Lashkar-e-Taiba, and Jundullah.”
Ms Sangwan explained that ‘313’ refers to the number of companions said to have fought with the Prophet Mohammed in the Battle of Badr.
TRAC have seen a recent uptick in TikTok videos and other social media posts that refer to ‘313’.
Many of the accounts are linked to each other.
Ms Sangwan said: “They [the TikTok users] mostly use ‘313’ as a hashtag… trying to push that hashtag to as many people it can reach on social media.”
Sky News sought to verify the location by comparing before and after videos from the strike location, and using the video released by the Indian army conducting the strike.
One video showing damage at the strike location was posted by a user with 313 in their TikTok username.
Image: The TikTok account that posted video footage of the destruction in Muridke has 313 in the username
Below is satellite imagery that shows the destruction of the site.
Image: Satellite imagery shows Markaz Taiba Mosque after the strike on 7 May. Credit: Maxar
In one TikTok, the video is captioned “bring your arms and ammunition and go to war”. The text on the screen of the TikTok is ‘313’ and he is carrying a gun.
The group are comfortable with having an online presence. On the Google tag for Markaz Taiba Mosque in Muridke, men pose for a group photo. Almost all the people in the photo have used ‘313’ on TikTok.
Ms Sangwan explained: “With these people from Muridke, pushing this propaganda on social media would generate a lot of significance in terms of recruitment and in terms of gaining support from local people and from other people.”
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Anger in Pakistan after India strikes
India says it struck Markaz Taiba, a site in Muridke about 15 miles (25km) from the border, which has long been claimed to be a terrorist training site associated with LeT.
MEMRI, a US-based research group that monitors terrorist threats, told Sky News: “It has been known for decades that Lashkar-e-Taiba has its headquarters in Muridke.”
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Sky News contacted the Pakistan Ministry of Defence for comment. Khawaja Asif, Pakistan’s defence minister, told Sky News: “This appears to be a random video with background music added later – consistent with how TikTok trends often function. If this is to be considered credible evidence, we could produce millions of similar clips ourselves.”
Mr Asif also said that any suggestion that the mosque was used as a base by terrorists was a “completely false, social media made up hoax”.
On 7 May, after the strikes in Pakistan, the Indian subcontinent branch of al Qaeda issued a statement condemning India’s actions and encouraging its supporters to wage jihad against India.
The Data and Forensics team is a multi-skilled unit dedicated to providing transparent journalism from Sky News. We gather, analyse and visualise data to tell data-driven stories. We combine traditional reporting skills with advanced analysis of satellite images, social media and other open source information. Through multimedia storytelling we aim to better explain the world while also showing how our journalism is done.
Russia’s president has suggested fresh peace talks with Ukraine in Istanbul as part of “direct negotiations” but also “without preconditions”.
Vladimir Putin put forward the proposal as European leaders including Sir Keir Starmer threatened him with fresh sanctions if Russia failed to comply with an unconditional 30-day ceasefire starting on Monday.
Kremlin spokesperson Dmitry Peskov told CNN that Moscow will need to consider those terms.
Speaking at the Kremlin in the early hours of Sunday, Mr Putin did not directly address the 30-day ceasefire proposal but instead offered to restart peace talks Russia and Ukraine held in 2022.
Image: Vladimir Putin told reporters he was committed to talks. Pic: RIA Novosti/AP
“We propose the Kyiv authorities resume the negotiations they interrupted at the end of 2022… to resume direct negotiations… without any preconditions… to begin without delay next Thursday 15 May in Istanbul,” he said.
Russia’s own unilateral three-day ceasefire, declared for the 80th anniversary of victory over Nazi Germany, expired on Saturday, and Ukraine said Russian forces have repeatedly violated it.
Image: European leaders including Volodymyr Zelenskyy hold call with Donald Trump. Pic: Number 10
During the summit in Kyiv, European leaders secured the backing of Donald Trump after briefing him on the progress made on the so-called “coalition of the willing” plans in a 20-minute phone call.
“All of us here, together with the US, are calling Putin out,” said Sir Keir.
“So we are clear, all five leaders here – all the leaders of the meeting we just had with the coalition of the willing – an unconditional ceasefire, rejecting Putin’s conditions, and clear that if he turns his back on peace, we will respond.
“Working with President Trump, with all our partners, we will ramp up sanctions and increase our military aid for Ukraine’s defence to pressure Russia back to the table.”
Image: Sir Keir Starmer, Emmanuel Macron and Friedrich Merz travelling in the saloon car of a special train to Kyiv. Pic: Reuters
Image: Leaders arrive in Kyiv by train. Pic: PA
It comes after President Trump called for “ideally” a 30-day ceasefire between Kyiv and Moscow, and warned that if any pause in the fighting is not respected “the US and its partners will impose further sanctions”.
During Mr Putin’s statement on Sunday, he insisted he would support peace talks: “We are committed to serious negotiations with Ukraine.”
He told reporters: “Their purpose is to eliminate the root causes of the conflict, to establish a long-term, lasting peace. We do not rule out that during these negotiations it will be possible to agree on some new truces, a new ceasefire.”
Putin cobbles together response to 30-day ceasefire demand
The Kremlin billed this as a meaningful statement from Vladimir Putin but how much does it actually mean?
His comments are exactly the same as what Moscow has been saying for weeks, only repackaged with a date. The latest attempt to avoid committing to a 30-day ceasefire.
Next Thursday – 15 May – is when he’s proposing to hold direct talks with Ukraine. Only then, he says, can they discuss the details of a longer truce.
Istanbul is apparently the venue. The trouble is, Turkey doesn’t appear to know about it. The Russian leader said he’d call President Erdogan tomorrow.
Given America’s apparent support for Europe’s ultimatum to Russia (ceasefire or sanctions), he had to respond with something new.
But this felt like a proposal that had been hastily cobbled together in the corridor outside.
Security and defence analyst Michael Clarke told Sky News presenter Samantha Washington the European leaders are “rowing in behind” the US president, who referred to his “European allies” for the first time in this context in a post on his Truth Social platform.
“So this meeting is all about heaping pressure on the Russians to go along with the American proposal,” he said.
“It’s the closest the Europeans and the US have been for about three months on this issue.”
Image: Trump calls for ceasefire. Pic: Truth Social
Mr Zelenskyy told reporters the agreed ceasefire should cover air, sea and land, and said that if Moscow refused, Russia would face new sanctions, including the strengthening of punitive measures targeting its energy and banking sectors.
The European leaders said the terms of a peace deal would be negotiated during the 30-day pause in fighting.
But the Ukrainian president said: “We have no illusions that the ceasefire will be breached.”
Mr Macron said the proposed ceasefire would be monitored mainly by the US and European countries and there would be “massive” sanctions if Russia did not agree.
Image: Sir Keir and Volodymyr Zelenskyy during a meeting in March. Pic: AP
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Putin’s Victory Day parade explained
Military officers from around 30 countries have been involved in drawing up plans for a coalition, which would provide a peacekeeping force in the event of a ceasefire being agreed between Russia and Ukraine.
This force “would help regenerate Ukraine’s armed forces after any peace deal and strengthen confidence in any future peace”, according to Number 10 Downing Street.
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Mr Peskov accused European leaders of making contradictory and confrontational statements, according to Interfax news agency.
“We hear many contradictory statements from Europe. They are generally confrontational in nature rather than aimed at trying to revive our relations. Nothing more,” he was quoted as saying.
Pope Leo XIV made his first outing since his election on Saturday, making a surprise stop to pray at the tomb of his predecessor.
On Saturday afternoon, the new pontiff travelled to a sanctuary dedicated to the Madonna in the town of Genazzano.
The sanctuary is managed by Augustinian friars, the order the pope belongs to, and has been a place of pilgrimage since the 15th century.
The pontiff’s namesake. Pope Leo XIII, elevated it to a minor basilica and expanded its convent in the early 1900s.
Image: Pope Leo XIV is cheered by residents of Genazzano , near Rome on 10 May. Pic: Vatican Media/AP
After praying, Leo greeted the faithful gathered outside and offered a blessing.
On his way back to the Vatican, he stopped to pray at Pope Francis’s tomb at St Mary Major Basilica.
Earlier in the day, Leo held his first formal audience and said the Catholic Church must take the lead in facing threats to workers, such as AI.
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The 69-year-old said the technology posed “new challenges for the defence of human dignity, justice and labour”.
Image: The Pope identified AI as a major challenge to humanity. Pic: Vatican Media/AP
The leader of the world’s 1.4 billion Catholics referred to AI as he explained his choice of name to the cardinals who elected him.
He said he identified with his namesake Pope Leo XIII, who was pontiff from 1878 to 1903 and addressed workers’ rights and capitalism at the dawn of the industrial age in the 1891 encyclical Rerum Novarum.
The late pope, who laid the foundation for modern Catholic social thought, criticised both laissez-faire capitalism and state-centric socialism.
“In our own day, the church offers everyone the treasury of its social teaching in response to another industrial revolution and to developments in the field of artificial intelligence that pose new challenges for the defence of human dignity, justice and labour,” Leo said.
The Pope also made clear he will follow in the modernising reforms of his predecessor Pope Francis to make the Catholic Church inclusive, attentive to the faithful, and an institution that looks out for the “least and rejected”.
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Leo said he was fully committed to the reforms of the Second Vatican Council, the 1960s meetings that modernised the church.
Toward the end of his pontificate, Francis became increasingly vocal about the threats to humanity posed by AI and called for an international treaty to regulate it.
Francis brought his message to the G7 summit of world leaders last year, insisting AI must remain human-centric so decisions about when to use weapons or even less-lethal tools always remain made by humans and not machines.