The four of them had taken a boat and gone for a swim at Saint Nikolas Beach – however, Dr Mosley preferred to return on foot at around 1.30pm, Athens-based newspaper Kathimerini reported.
The CCTV images were captured by a restaurant at around 2pm local time, police believe.
The beachside eatery is located in the Pedi area of the island, a small town in a bay around 1.2km from Saint Nikolas Beach.
The images appear to show the 67-year-old wearing a dark t-shirt, shorts and a cap. He is carrying an umbrella.
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Dr Mosley, his wife and the other couple arrived in Symi for a week’s holiday on Tuesday, according to local media.
The doctor had been missing for just over 48 hours, as of Friday afternoon.
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Symi has been experiencing hot temperatures this week and its mayor Lefteris Papakalodoukas told a local news site the heat on Wednesday was “unbearable and one could easily faint in such conditions”.
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The rescue operation has been focusing on the Pedi area after a woman reported seeing him there on Wednesday.
The extensive search has so far seen five coastguard patrol boats deployed while private and commercial vessels in the area have also helped.
Police and firefighters have used drones to scan the island and a helicopter was deployed at around 7pm local time on Thursday. Sniffer dogs have also been used in the search.
Divers have been looking in the water, Symi deputy mayor Ilias Chaskas previously said.
Authorities have considered a number of possibilities, including Dr Mosley may have suffered a fall – or could have been bitten by a snake, Greek news website ekathimerini.com reported.
One woman in the Pedi area said the TV doctor’s disappearance was “strange” as the path he was thought to be on is “clear”.
“It’s a quiet place,” she said. “If you see the map of the area it’s a clear path, it’s nothing dangerous.
“Many people go every day, every few minutes, that’s the reason it’s very strange because it’s a clear path.”
Dr Mosley is a columnist and broadcaster who has made a number of films about diet and exercise.
He fronted the Channel 4 show Michael Mosley: Who Made Britain Fat? and was part of the BBC series Trust Me, I’m A Doctor. He has also appeared on numerous daytime television shows.
Conservative Party leader Kemi Badenoch has called on Sir Keir Starmer to sack Treasury minister Tulip Siddiq over allegations she lived in properties linked to allies of her aunt, Sheikh Hasina, the deposed prime minister of Bangladesh.
It comes after the current Bangladeshi leader, Muhammad Yunus, said London properties used by Ms Siddiq should be investigated.
He told the Sunday Timesthe properties should be handed back to his government if they were acquired through “plain robbery”.
Tory leader Ms Badenoch said: “It’s time for Keir Starmer to sack Tulip Siddiq.
“He appointed his personal friend as anti-corruption minister and she is accused herself of corruption.
“Now the government of Bangladesh is raising serious concerns about her links to the regime of Sheikh Hasina.”
Ms Siddiq insists she has “done nothing wrong”.
Her aunt was ousted from office in August following an uprising against her 20-year leadership and fled to India.
On the same day, the prime minister said: “Tulip Siddiq has acted entirely properly by referring herself to the independent adviser, as she’s now done, and that’s why we brought into being the new code.
“It’s to allow ministers to ask the adviser to establish the facts, and yes, I’ve got confidence in her, and that’s the process that will now be happening.”
Police in Aberdeen have widened the search area for two sisters who disappeared four days ago in the city.
Eliza and Henrietta Huszti, both 32, were last seen on CCTV on Market Street after leaving their home on Tuesday at around 2.12am.
The sisters – who are part of a set of triplets and originally from Hungary – crossed the Victoria Bridge to the Torry area and turned right on to a footpath next to the River Dee.
They headed in the direction of Aberdeen Boat Club but officers said there is no evidence to suggest the missing women left the immediate area.
Specialist search teams, police dogs and a marine unit have been trying to trace the pair.
Further searches are being carried out towards the Port of Aberdeen’s South Harbour and Duthie Park.
Police Scotland said it is liaising with authorities in Hungary to support the relatives of the two sisters.
Chief Inspector Darren Bruce said: “Eliza and Henrietta’s family are understandably extremely worried about them and we are working tirelessly to find them.
“We are seriously concerned about them and have significant resources dedicated to the inquiry.”
The sisters, from Aberdeen city centre, are described as slim with long brown hair.
Officers have requested businesses in and around the South Esplanade and Menzies Road area to review their CCTV footage for the early morning of Tuesday 7 January.
Police added they are keen to hear from anyone with dashcam footage from that time.
TV presenter Katie Piper has revealed her decision to get an artificial eye, 16 years after an acid attack that left her with life-changing injuries and partial blindness.
The Loose Women panellist, 41, is an advocate for those with burns and disfigurement injuries.
She shared a video of her being fitted with the prosthetic on Instagram.
Piper said: “After many years battling with my eye health, I’ve reached the end of the road somewhat, and the decision has been made to try a prosthetic eye shell.
“This marks the start of a journey to have an artificial eye, with an incredible medical team behind me.
“As always I’m incredibly grateful to all those in the NHS and private health care system for their talent and kindness.
“I will share my journey, I’m hopeful and nervous about being able to tolerate it and would love to hear from any of you in the comments if you’ve been on this journey or have any advice.”
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Commenting on the post, presenter Lisa Snowdon said Piper was a “warrior” and a “true inspiration”.
Piper has undergone hundreds of operations after suffering an acid attack arranged by her ex-boyfriend in March 2008.
She gave up her right to anonymity and made a documentary in 2009 called Katie: My Beautiful Face.
Piper also founded the Katie Piper Foundation which supports survivors of life-changing burns and scars, and has received an honorary doctorate from the Royal College of Surgeons to mark her ground-breaking work.
She was made an OBE in 2021 for her services to charity and burn victims.