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Researchers have introduced an advanced method for labeling proteins in intact tissues, addressing a longstanding challenge in biological research. The technique, designed to ensure even distribution of antibodies across entire organs, has been demonstrated across multiple tissue types. This approach could significantly improve the accuracy of cellular studies by preserving tissue structure while enabling more precise protein detection. Scientists expect the method to aid in the study of complex biological systems, providing insights into cellular functions without altering the natural architecture of tissues.

Breakthrough in Protein Labeling

According to a study published in Nature Biotechnology, the technique, known as continuous redispersion of volumetric equilibrium (CuRVE), was developed at the Massachusetts Institute of Technology (MIT). The research team, led by Kwanghun Chung, Associate Professor of Chemical Engineering and Neuroscience at MIT, tested the method on various biological samples, including rodent and human tissues. By controlling the speed of antibody-antigen interactions and enhancing molecular diffusion through stochastic electrotransport, the process allowed for uniform protein labeling in a fraction of the time required by conventional methods.

Addressing Limitations of Conventional Methods

Traditional approaches such as immunohistochemistry often fail to achieve uniform protein distribution due to the size of labeling molecules. The antibodies tend to concentrate near the surface while failing to penetrate deeper layers, leading to uneven labeling. Speaking to MIT News, Chung compared the challenge to marinating a thick cut of meat, where only the outer layers absorb the marinade effectively. He explained that the large size of labeling molecules makes uniform penetration extremely difficult, requiring prolonged processing times.

Efficient and Scalable Application

The researchers demonstrated that their CuRVE technique, combined with electrophoretic-fast labeling using affinity sweeping in hydrogel (eFLASH), significantly improved antibody penetration. Tests on an adult mouse brain showed that conventional methods resulted in uneven labeling, while eFLASH produced uniform staining across all neurons. Similar success was observed in tissues from other species, including marmoset and human brain samples, as well as mouse embryos, lungs, and hearts.

Comparison with Genetic Labeling

As reported by The Scientist, the key advantage of this technique over transgenic labeling was highlighted in the study. While genetic methods rely on fluorescent markers linked to gene transcription, protein expression does not always correlate directly with gene activity. Researchers found significant discrepancies when comparing transgenic labeling with eFLASH, emphasizing the need for direct protein detection. The findings suggest that genetic labeling alone may not be sufficient for accurately studying protein distribution in tissues.

Future Implications

Scientists anticipate that CuRVE will contribute to the creation of a comprehensive repository of protein expression patterns across different tissue types. This could serve as a reference for studying diseased tissues and refining diagnostic methods. Researchers believe that by improving protein visualization at the cellular level, the technique may enhance the understanding of various biological processes, potentially influencing future medical and scientific applications.

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Japan Launches Final H-2A Rocket with GOSAT-GW Satellite to Monitor Climate and Oceans

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July 1, 2025

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Japan Launches Final H-2A Rocket with GOSAT-GW Satellite to Monitor Climate and Oceans

Japan launched a satellite which is a dual purpose, for monitoring greenhouse gases and sea temperature. On Saturday, June 28, 2025, the Japan Aerospace Exploration Agency (JAXA) held its 50th and final launch of the H-2A rocket, launching the GOSAT-GW satellite of dual purpose to space. This mission lifted off from Ypshinobu Launch Complex (LP-1) in Japan at Tenegashima Space Centre at 10:03 p.m. IST. These satellites have been sent by Japan to observe the changes in water cycles and greenhouse gases.

JAXA’s Dual Purpose Satellite Launch

According to JAXA, the Greenhouse Gas and Water Cycle Observation Satellite (GOSA-GW) is a very recent and significant effort of Japan to know about the effect of greenhouse gases and the changes in the cycle of rain. GOSAT-GW has joined the orbit of Earth with GCOM-W2, which is its predecessor, and was launched in 2012, known as SHIZUKU, whereas GOSAT-1, launched in 2009, is known as IBUKI. It is equipped with two main instruments.

More About GOSAT-GW

GOSAT-GW is equipped with two main instruments, the first is called the Advanced Microwave Radiometer (AMSR), and the other is known as the Greenhouse Gases Observation Sensor (TANSO). The former one will measure water cycles and sea surface temperature fluctuations, while the latter one is for monitoring components such as carbon dioxide in Earth’s atmosphere for measuring climate change.

H-2A rocket of Japan has been capable of launching payloads into the geostationary orbit around Earth and also orbiting the Moon. JAXA also launched the Akatsuki spacecraft for studying Venus in 2010; however, the spacecraft failed to enter the orbit of Venus properly.

Japan’s Successful Launch Operations

JAXA launched H-2A for the first time in 2001. With its 25 years in operation, the rocket experienced only a single failure, giving the spacecraft a 98% success rate. After 50 missions, the launch vehicle is now being retired to make way for the H3 rocket of Japan, offering comparable performance at a very reasonable cost.

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SpaceX Launches 53 Starlink Satellites in One Day, Crossing 7,900 Active Units

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July 1, 2025

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SpaceX Launches 53 Starlink Satellites in One Day, Crossing 7,900 Active Units

On June 28, 2025, two Falcon 9 rockets carrying the internet satellite broadband connection lifted off at 12:26 a.m. and 1:13 p.m. EDT. The first one was launched from Space Launch Complex 40 at Cape Canaveral Space Force Station, Florida, and another from Space Launch Complex 4 East at Vandenberg Space Force Base, California. The new addons for megaconsellation of SpaceX (Group 10-34 and Group 15-7) reached low orbit Earth about 9 minutes after leaving the ground and were deployed after an hour.

According to As per NASA, in the intervening time, the first stage of the Falcon rockets (boosters B1092 and B1088) flew back to the droneships. The landing was on “A Shortfall of Gravitas, “ completing the fifth flight of the boosters. The stage touched down on “Of Course I Still Love You”, with its eighth flight to space and back, in the Pacific Ocean.

The launch at early morning carries 27 Starlink Satellites and the afternoon liftoff lofted 26 more of the relay satellites, vasting the network of SpaceX to more than 7,900 active units, said by satellite tracker Jonathan Mc Dowell.

Broader Deployment Context

The launch in the morning was delayed earlier that day because of the severe weather alerts at Florida, but flew in the near perfect conditions at the visibility of 10 miles. Throughout 2025, SpaceX heald 42 Falcon 9 missions with 28 Starlink launched by June. by mid-June the batch of 26 satellites launched from Vandenberg (Booster B1081’s 15th flight) which contributed to more than 7,600 active satellites by June 12.

Both the missions are a part of an aggressive Starlink deployment, mainly for boosting the low Earth orbit constellation for the global broadband coverage. Now the network expands to approximately 7900 satellites, focusing on the biggest LEO broadband constellation ever.

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Astronomers Discover a Gigantic Supernova Remnant in the Large Magellanic Cloud

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July 1, 2025

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Astronomers Discover a Gigantic Supernova Remnant in the Large Magellanic Cloud

Astronomers international team has employed various satellites and telescopes for performing multiwavelength observations of a supernova remnant known as SNR J0450.4-7050. The result that scientists got after an observational campaign, published on June 18, 2025, on the preprint server arXiv, gained new insights into the properties of this remnant, and observed that it is much larger than thought in the past. Supernova remnants (SNRs) are expanded structures resulting from the explosion of a supernova. It usually lasts for several hundred thousand years before the time it disperses into the interstellar medium.

Multiwavelength Observations Reveal New Details of SNR J0450.4-7050

As per the study by NASA, observations by the scientists indicate that SNRs contain the ejected expansion of material from the explosion and other interstellar material swept by the passage of the shockwave from the star that exploded. SNR studies beyond the Milky Way are important for comprehending the feedback in different evolutionary phases and gaining valuable insights into the local ISM. The Large Magellanic Cloud is the galaxy that has its SNR population explored in depth.

Importance of Studying Supernova Remnants Beyond the Milky Way

The recent observations reveal that SNR J0450.4-7050, a large supernova remnant in the LMC having physical dimensions of 332 by 244 light years, is estimated to have the remnants of around 45,000 years old. Scientists named it Veliki, which means large in Serbian. It also showcases the complex filamentary morphology with different inner and outer shell structures.

Discovery of Veliki: A Large, Aged Remnant in the Large Magellanic Cloud

An astronomer’s group led by Zachary J. Smeaton decided to take a closer observation of SNR J0450.4−7050 by the Australian Square Kilometre Array Pathfinder and MeerKAT radio telescope. The SNR showed a high radial surface brightness with one of the lowest average radio spectral indices, with similar remnants.

Unusual Radio Properties Suggest Veliki is a Fully Radiative SNR

These unusual features, according to the scientists, give the prediction that Veliki is mainly a radiative SNR with a high shock compression ratio. This states about the non-thermal spectrum and higher surface brightness and emissions. Further observations of the surrounding environment are needed to confirm the hypothesis with an understanding of the remnants’ nature in a better way.

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